Annatto extract is a natural food color obtained from the outer coatings of the seeds of the Annatto tree (Bixa orellana L.). Annatto extract is a natural food color, which is obtained from the outer coatings of the seeds of the Annatto tree (Bixa orellana L) The use of annatto by the New World man dates back to ancient times. The indigenous Amazon people have used ?urucum? for body painting for centuries. Seeds and leaves of the annatto tree were used by the Aztecs to prepare remedies for a variety of illnesses such as tonsillitis, asthma, pleurisy, rectal disorders, headache, jaundice, sunstroke, and burns. Bixin is used both as a spice and a dye since ancient time. Color is the first sensory quality by which foods are judged, since food quality and flavor are closely associated with color. In addition, many colorless convenience foods such as confectionary products, snacks, beverages and gelatin desserts, the food color was added in order to identify the taste and increased the palatability.